کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936150 1050684 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
La autoantigen suppresses IRES-dependent translation of the hepatitis A virus
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
La autoantigen suppresses IRES-dependent translation of the hepatitis A virus
چکیده انگلیسی

The human RNA-binding protein La, is an essential trans-acting factor in IRES-dependent translation initiation of poliovirus, the prototypic picornavirus. For hepatitis A virus (HAV), an unusual member of this virus family, the role of host proteins in its inefficient translation and slow replication is unclear. Using small interfering RNA in vivo and purified La in vitro, we demonstrate for the first time that La suppresses HAV IRES-mediated translation and replication. We show that La binds specifically to distinct parts of the HAV IRES and that—unlike poliovirus—HAV proteinase 3C does not cleave La. The La-mediated suppression of HAV translation and stimulation of poliovirus translation implies unexpected mechanistic differences between viral IRES elements.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 368, Issue 4, 18 April 2008, Pages 1014–1019
نویسندگان
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