کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936752 1536791 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli
چکیده انگلیسی

The bacterial chemotaxis adaptor protein CheW physically links the chemoreceptors (MCPs) and the histidine kinase CheA. Extensive investigations using bacterium Escherichia coli have established the central role of CheW in the MCP-modulated activation of CheA. Here we report the solution structure of CheW from E. coli determined by NMR spectroscopy. The results show that E. coli CheW shares an overall fold with previously reported structure of CheW from Thermotoga maritima, whereas local conformational deviations are observed. In particular, the C-terminal α-helix is considerably longer in E. coli CheW and appears to shrink the active binding pocket with CheA. Our study provides the structural basis for further investigations in E. coli chemotaxis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 360, Issue 4, 7 September 2007, Pages 863–867
نویسندگان
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