کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1936790 | 1050702 | 2008 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Glycosylation of the OMP85 homolog of Porphyromonas gingivalis and its involvement in biofilm formation Glycosylation of the OMP85 homolog of Porphyromonas gingivalis and its involvement in biofilm formation](/preview/png/1936790.png)
OMP85 is a highly conserved outer membrane protein in all Gram-negative bacteria. We studied an uncharacterized OMP85 homolog of Porphyromonas gingivalis, a primary periodontal pathogen forming subgingival plaque biofilms. Using an outer-loop peptide antibody specific for the OMP85 of P. gingivalis, loop-3 Ab, we found a difference in the mobility of OMP85 on SDS–PAGE gel between the P. gingivalis wild-type and the isogenic galE mutant, a deglycosylated strain, suggesting that OMP85 naturally exists in a glycosylated form. This was also supported by a shift in OMP85 PAGE mobility after chemical deglycosylation treatment. Further, loop-3 Ab cross-reacted with the galE mutant stronger than the wild-type strain; and could inhibit biofilm formation in the galE mutant more than in the wild-type strain. In conclusion, this is the first report providing the evidence of OMP85 glycosylation and the involvement of OMP85 in biofilm formation.
Journal: Biochemical and Biophysical Research Communications - Volume 365, Issue 4, 25 January 2008, Pages 784–789