کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936870 1050704 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Brain region-specific N-glycosylation and lipid rafts association of the rat mu opioid receptor
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Brain region-specific N-glycosylation and lipid rafts association of the rat mu opioid receptor
چکیده انگلیسی

The mu opioid receptor (MOR) in the rat and mouse caudate putamen (CPu) and thalamus was demonstrated as diffuse and broad bands by Western blot with a polyclonal antibody against a C-terminal peptide of MOR, which were absent in the cerebellum and brains of MOR-knockout mice. The electrophoretic mobility of MOR differed in the two brain regions with median relative molecular masses (Mr’s) of 75 kDa (CPu) vs. 66 kDa (thalamus) for the rat, and 74 kDa (CPu) vs. 63 kDa (thalamus) for the mouse, which was due to its differential N-glycosylation. Rat MOR in CPu was found mainly associated with low-density cholesterol- and ganglioside M1 (GM1)-enriched membrane subdomains (lipid rafts), while the MOR in the thalamus was present in rafts and non-rafts without preference. Cholesterol reduction by methyl-β-cyclodextrin decreased DAGMO-induced [35S]GTPγS binding in rat CPu membranes to a greater extent than in the thalamus membranes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 365, Issue 1, 4 January 2008, Pages 82–88
نویسندگان
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