کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936990 1050706 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultra-high field NMR studies of antibody binding and site-specific phosphorylation of α-synuclein
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Ultra-high field NMR studies of antibody binding and site-specific phosphorylation of α-synuclein
چکیده انگلیسی

Although biological importance of intrinsically disordered proteins is becoming recognized, NMR analyses of this class of proteins remain as tasks with more challenge because of poor chemical shift dispersion. It is expected that ultra-high field NMR spectroscopy offers improved resolution to cope with this difficulty. Here, we report an ultra-high field NMR study of α-synuclein, an intrinsically disordered protein identified as the major component of the Lewy bodies. Based on NMR spectral data collected at a 920 MHz proton frequency, we performed epitope mapping of an anti-α-synuclein monoclonal antibody, and furthermore, characterized conformational effects of phosphorylation at Ser129 of α-synuclein.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 363, Issue 3, 23 November 2007, Pages 795–799
نویسندگان
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