کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937035 1050707 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A role for His155 in binding of human prion peptide144–167 to immobilised prion protein
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A role for His155 in binding of human prion peptide144–167 to immobilised prion protein
چکیده انگلیسی

The interactions and conformational changes that lead to the conversion of the normal prion protein (PrPc) to its pathogenic form, PrPsc, are still being elucidated. Using Surface Plasma Resonance (SPR), we provide evidence that a synthetic peptide (PrP144–167) corresponding to residues comprising the α helix 1–β strand 2 domain of PrPc is able to interact and bind to immobilised recombinant human PrP (rHuPrP) in a dose-dependent manner. The interaction is pH dependent with an increase in binding observed as the pH is lowered, particularly between pH 6.5 and pH 5.5 suggesting a specific role for His155 in the interaction, confirmed by covalent modification of this residue in the peptide with diethylpyrocarbonate (DEPC). Circular dichroism analysis of PrP144–167 revealed no secondary structure motifs across the pH range investigated. Possible pH related structural changes of immobilised rHuPrP are also discussed with regard to the increased affinity for PrP144–167.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 362, Issue 3, 26 October 2007, Pages 695–699
نویسندگان
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