کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1938075 1050731 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cellular prion protein in ovine milk
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Cellular prion protein in ovine milk
چکیده انگلیسی

Cellular prion protein, PrPC, is essential for the development of prion diseases where it is considered to be a substrate for the formation of the disease-associated conformer, PrPSc. In sheep, PrPC is abundant in neuronal tissue and is also found at lower concentrations in a range of non-neuronal tissues, including mammary gland. Here, we demonstrate the presence of soluble PrPC in the non-cellular, non-lipid fraction of clarified ovine milk. Compared with brain-derived PrPC, ovine milk PrPC displays an increased electrophoretic mobility. Ovine milk PrPC is mainly present as three species that differ in the extent of their N-linked glycosylation, with glycoform profiles varying among animals. Similar PrPC species are also present in fresh and commercial homogenised/pasteurised bovine milk, with additional N-terminal PrPC fragments detectable in ruminant milk and commercial milk products.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 353, Issue 1, 2 February 2007, Pages 195–199
نویسندگان
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