کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1938812 1050747 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot
چکیده انگلیسی

In order to study the multiple functions of fibrinogen and fibrin, we are investigating which proteins bind to the fibrin matrix of a plasma clot by using a proteomic approach. Extracts from washed plasma clots were analysed by 2-D gel electrophoresis. A relatively abundant spot was identified as hepatocyte-derived fibrinogen-related protein-1 (HFREP-1) by MALDI-TOF analysis, molecular mass (34 kDa), iso-electric point (pI 5.5) as well as by Western blot analysis. HFREP-1 in plasma almost completely bound to the fibrin matrix during clot formation. Several purified fibrinogen preparations proved to be contaminated with HFREP-1. It is concluded that HFREP-1 (also named hepassocin), a protein with liver cell growth regulatory properties, occurs in plasma and strongly associates with fibrin and possibly fibrinogen.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 350, Issue 1, 10 November 2006, Pages 191–194
نویسندگان
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