کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1941086 | 1050797 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi](/preview/png/1941086.png)
چکیده انگلیسی
Trypanosoma cruzi, the etiological agent of Chagas’ disease, is the only eukaryotic cell which lacks the ability to synthesize polyamines de novo. In this work, we describe for the first time the molecular and biochemical properties of a high-affinity spermidine transporter from T. cruzi. The transporter gene TcPAT12 was functionally expressed in Xenopus laevis oocytes, showing high levels of spermidine uptake. Similar apparent affinity constants for spermidine uptake were obtained when comparing T. cruzi epimastigotes and heterologous expressed TcPAT12 in X. laevis. In addition, TcPAT12 also transports putrescine and the amino acid l-arginine at lower rates than spermidine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 344, Issue 3, 9 June 2006, Pages 936–940
Journal: Biochemical and Biophysical Research Communications - Volume 344, Issue 3, 9 June 2006, Pages 936–940
نویسندگان
Carolina Carrillo, Gaspar E. Canepa, Israel D. Algranati, Claudio A. Pereira,