کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1942169 | 1052585 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic characterization and three-dimensional electron microscopy study of PSII-LHCII supercomplexes from higher plants
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کلمات کلیدی
OECLMMBN-PAGEChlFSCThree-dimensionalThylakoidsβ-DMPTMLC-ESI-MS/MS - LC-ESI-MS / MSMALDI-TOF/TOF - MALDI-TOF / TOFn-Dodecyl-β-D-maltoside - n-Dodecyl-β-D-مالتوزیدfield emission gun - اسلحه انتشار میدانPost translational modification - اصلاح پست ترجمهblue native polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمید آبی بومیOxygen evolving complex - اکسیژن تکامل یافته پیچیده استTem - این استLHC - برخورد دهنده بزرگ هادرون Single particle analysis - تجزیه و تحلیل ذرات تکlow molecular mass - توده مولکولی کمStructure - ساختار یا سازهChlorophyll - سبزینه یا کلروفیلPhotosystem - سیستم عکسMass spectrometry - طیف سنجی جرمیLight harvesting complex - مجتمع برداشت نورfeG - مرغReaction centre - مرکز واکنشTransmission electron microscopy - میکروسکوپ الکترونی عبوریFourier shell correlation - همبستگی پوسته فوریهProteomics - پروتئومیکسLiquid chromatography electrospray ionization tandem mass spectrometry - کروماتوگرافی مایع الکترو اسپری یونیزاسیون اسپکترومتری دو طرفه
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In higher plants a variable number of peripheral LHCII trimers can strongly (S), moderately (M) or loosely (L) associate with the dimeric PSII core (C2) complex via monomeric Lhcb proteins to form PSII-LHCII supercomplexes with different structural organizations. By solubilizing isolated stacked pea thylakoid membranes either with the α or β isomeric forms of the detergent n-dodecyl-D-maltoside, followed by sucrose density ultracentrifugation, we previously showed that PSII-LHCII supercomplexes of types C2S2M2 and C2S2, respectively, can be isolated [S. Barera et al., Phil. Trans. R Soc. B 67 (2012) 3389-3399]. Here we analysed their protein composition by applying extensive bottom-up and top-down mass spectrometry on the two forms of the isolated supercomplexes. In this way, we revealed the presence of the antenna proteins Lhcb3 and Lhcb6 and of the extrinsic polypeptides PsbP, PsbQ and PsbR exclusively in the C2S2M2 supercomplex. Other proteins of the PSII core complex, common to the C2S2M2 and C2S2 supercomplexes, including the low molecular mass subunits, were also detected and characterized. To complement the proteomic study with structural information, we performed negative stain transmission electron microscopy and single particle analysis on the PSII-LHCII supercomplexes isolated from pea thylakoid membranes solubilized with n-dodecyl-α-D-maltoside. We observed the C2S2M2 supercomplex in its intact form as the largest PSII complex in our preparations. Its dataset was further analysed in silico, together with that of the second largest identified sub-population, corresponding to its C2S2 subcomplex. In this way, we calculated 3D electron density maps for the C2S2M2 and C2S2 supercomplexes, approaching respectively 30 and 28 Ã
resolution, extended by molecular modelling towards the atomic level. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1837, Issue 9, September 2014, Pages 1454-1462
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1837, Issue 9, September 2014, Pages 1454-1462
نویسندگان
Cristina Pagliano, Jon Nield, Francesco Marsano, Tillmann Pape, Simone Barera, Guido Saracco, James Barber,