کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1944881 | 1537145 | 2009 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential endocytic trafficking of neuropathy-associated antibodies to GM1 ganglioside and cholera toxin in epithelial and neural cells
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کلمات کلیدی
DMEMGalNAc-TTGNYFPCTXCTXBGangliosideGBSFRAPGFPPBSFBSTransferrinEndocytic recycling - بازیافت اندوسیتیکCho - برایChinese Hamster Ovary - تخمدان هامستر چینیRoom temperature - دمای اتاقfetal bovine serum - سرم جنین گاوGuillain–Barré syndrome - سندرم Guillain-Barrécholera toxin - سوزش گوارشیTrans-Golgi network - شبکه Trans-Goljiendoplasmic reticulum - شبکه آندوپلاسمی Plasma membrane - غشای پلاسماfluorescence recovery after photobleaching - فلوئورسانس پس از فوتوبلاسیکIntracellular trafficking - قاچاق داخل سلولیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریyellow fluorescent protein - پروتئین فلورسنت زردgreen fluorescent protein - پروتئین فلورسنت سبزcholera toxin B subunit - کلسترول توکسین B واحد
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Gangliosides are glycolipids mainly present at the plasma membrane (PM). Antibodies to gangliosides have been associated with a wide range of neuropathy syndromes. Particularly, antibodies to GM1 ganglioside are present in patients with Guillain-Barré syndrome (GBS). We investigated the binding and intracellular fate of antibody to GM1 obtained from rabbits with experimental GBS in comparison with the transport of cholera toxin (CTx), which binds with high affinity to GM1. We demonstrated that antibody to GM1 is rapidly and specifically endocytosed in CHO-K1 cells. After internalization, the antibody transited sorting endosomes to accumulate at the recycling endosome. Endocytosed antibody to GM1 is recycled back to the PM and released into the culture medium. In CHO-K1 cells, antibody to GM1 colocalized with co-endocytosed CTx at early and recycling endosomes, but not in Golgi complex and endoplasmic reticulum, where CTx was also located. Antibody to GM1, in contraposition to CTx, showed a reduced internalization to recycling endosomes in COS-7 cells and neural cell lines SH-SY5Y and Neuro2A. Results from photobleaching studies revealed differences in the lateral mobility of antibody to GM1 in the PM of analyzed cell lines, suggesting a relationship between the efficiency of endocytosis and lateral mobility of GM1 at the PM. Taken together, results indicate that two different ligands of GM1 ganglioside (antibody and CTx) are differentially endocytosed and trafficked, providing the basis to gain further insight into the mechanisms that operate in the intracellular trafficking of glycosphingolipid-binding toxins and pathological effects of neuropathy-associated antibodies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1788, Issue 12, December 2009, Pages 2526-2540
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1788, Issue 12, December 2009, Pages 2526-2540
نویسندگان
Ramiro Iglesias-Bartolomé, Alejandra Trenchi, Romina ComÃn, Ana L. Moyano, Gustavo A. Nores, Jose L. Daniotti,