Assembling chromatin: The long and winding road

It has been over 35 years since the acceptance of the “chromatin subunit” hypothesis, and the recognition that nucleosomes are the fundamental repeating units of chromatin fibers. Major subjects of inquiry in the intervening years have included the steps involved in chromatin assembly, and the chaperones that escort histones to DNA. The following commentary offers an historical perspective on inquiries into the processes by which nucleosomes are assembled on replicating and nonreplicating chromatin. This article is part of a Special Issue entitled: Histone chaperones and Chromatin assembly.

► An historical perspective of the chromatin assembly field is presented.
► Early investigations into nucleosome assembly and histone segregation are discussed.
► Initial attempts to identify chromatin assembly factors are highlighted.