کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1948729 | 1054708 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of mitochondrial morphology and cell survival by Mitogenin I and mitochondrial single-stranded DNA binding protein
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کلمات کلیدی
PBSDMEMFDDfission/fusionGFPmtSSBeGFPenhanced GFPDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccojuvenile visceral steatosis - استئاتوز عصبی نوجوانانJVs - جاسوسیApoptosis - خزان یاختهایreverse transcription - رونویسی معکوسMitochondrial biogenesis - زیست زیستی میتوکندریاییMitochondria - میتوکندریاgreen fluorescent protein - پروتئین فلورسنت سبزPropidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Regulation of mitochondrial morphology and cell survival by Mitogenin I and mitochondrial single-stranded DNA binding protein Regulation of mitochondrial morphology and cell survival by Mitogenin I and mitochondrial single-stranded DNA binding protein](/preview/png/1948729.png)
چکیده انگلیسی
We found that a mouse homolog of human DNA polymerase delta interacting protein 38, referred to as Mitogenin I in this paper, and mitochondrial single-stranded DNA-binding protein (mtSSB), identified as upregulated genes in the heart of mice with juvenile visceral steatosis, play a role in the regulation of mitochondrial morphology. We demonstrated that overexpression of Mitogenin I or mtSSB increased elongated or fragmented mitochondria in mouse C2C12 myoblast cells, respectively. On the other hand, the silencing of Mitogenin I or mtSSB by RNA interference led to an increase in fragmented or elongated mitochondria in the cells, respectively, suggesting that Mitogenin I and mtSSB are involved in the processes of mitochondrial fusion and fission, respectively. In addition, we showed that the silencing of Mitogenin I resulted in an increase in the number of trypan blue-positive cells and the silencing of mtSSB resulted in an enhancement of the sensitivity of the cells to apoptotic stimulation by etoposide. The present results demonstrated that these proteins play a role in cell survival.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1760, Issue 9, September 2006, Pages 1364-1372
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1760, Issue 9, September 2006, Pages 1364-1372
نویسندگان
Naokatu Arakaki, Takeshi Nishihama, Akira Kohda, Hiroyuki Owaki, Yoshinori Kuramoto, Reika Abe, Toshiyuki Kita, Midori Suenaga, Toshiki Himeda, Masamichi Kuwajima, Hirofumi Shibata, Tomihiko Higuti,