کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1951063 1537961 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Caspase-mediated cleavage of importin-α increases its affinity for MCM and downregulates DNA synthesis by interrupting the binding of MCM to chromatin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Caspase-mediated cleavage of importin-α increases its affinity for MCM and downregulates DNA synthesis by interrupting the binding of MCM to chromatin
چکیده انگلیسی

Importin-α is essential for classical nucleocytoplasmic transport of nuclear proteins. Here, we report that importin-α is cleaved by caspases during apoptosis, generating importin-α lacking an IBB domain. This truncated importin-α binds tightly to the MCM replication licensing factor and, thus, prevents its binding to chromatin and downregulates DNA synthesis. Together, our data reveal for the first time that a dying cell effectively salvages limited supplies of cellular energy to ensure an orderly process of its own demise by simultaneously downregulating nucleocytoplasmic protein transport and DNA synthesis. Strikingly, cells can achieve this multi-task process by simply cleaving-off a key nuclear import protein.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1783, Issue 12, December 2008, Pages 2287–2293
نویسندگان
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