کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1953202 | 1057255 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mechanism of cytosol phospholipase C and sphingomyelinase-induced lysosome destabilization
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کلمات کلیدی
PLA2Ara-AMPethylene glycol-bis-(2-aminoethylether)-N,N,N′,N′-tetraacetic acidD609FITC-dextranPLDSMaseCCCPPLCEGTA[Ca2+] - [Ca2 +]phospholipase A2 - آنزیم فسفولیپاز A2 sphingomyelinase - اسپینگومایینازOxonol VI - اکسونول VICa2+ concentration - غلظت Ca2 +phospholipase C - فسفولیپاز CPhospholipase D - فسفولیپاز DFluorescein isothiocyanate-dextran - فلوورسسین ایزوتوسیانات-دکسترانLysosome - لیزوزومProton - پروتونpotassium ion - یون پتاسیمCalcium ion - یون کلسیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lysosomal disintegration may cause apoptosis, necrosis and some diseases. However, mechanisms for these events are still unclear. In this study, we measured lysosomal β-hexosaminidase free activity, membrane potential and intralysosomal pH. The results revealed that the cytosolic extracts of rat hepatocytes could increase the lysosomal permeability to both potassium ions and protons, and osmotically destabilize lysosomes via K+/H+ exchange. The effects of cytosol on lysosomes could be completely abolished by D609, which inhibited both phospholipase C and sphingomyelinase, and partly prevented by sphingomyelinase inhibitor Ara-AMP, but not by the inhibitors of PLA2. Moreover, purified phospholipase C could destabilize the lysosomes while phospholipase A2 and phospholipase D did not produce such effects. The cytosolic phospholipases hydrolyzed lysosomal membrane phospholipids by 50%, which could be prevented by D609. Disintegration of the cytosol-treated lysosomes biphasically depended on the cytosolic [Ca2+]. The cytosol did not disintegrate lysosomes below 100 nM or above 10 μM cytosolic [Ca2+], but markedly destabilized lysosomes at about 340 nM [Ca2+]. The results suggest that cytosolic phospholipase C and sphingomyelinase may be responsible for the alterations in lysosomal stability by increasing the ion permeability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 88, Issue 7, July 2006, Pages 913-922
Journal: Biochimie - Volume 88, Issue 7, July 2006, Pages 913-922
نویسندگان
Xiang Wang, Hui-Fang Zhao, Guo-Jiang Zhang,