کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
195385 459812 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a membrane-based electrochemical immunosensor
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Development of a membrane-based electrochemical immunosensor
چکیده انگلیسی

A membrane-based electrochemical immunosensor sensitive towards proteins is described, based on nanoporous alumina-modified platinum wire electrodes. The sensing mechanism depends on the blocking of pore channels when the protein antigen molecules bind to antibody molecules attached to the channel walls, impeding the diffusion of redox probe, ferrocenemethanol, towards the sensing platinum wire overlaid by the nanoporous alumina film. The antibody and antigen used in this work were anti-glucose oxidase and glucose oxidase, respectively. The immunosensor showed a low limit of detection of 100 ng L−1 antigen concentration and was selective towards glucose oxidase protein in the presence of another protein, glucothione S-transferase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 53, Issue 2, 1 December 2007, Pages 803–810
نویسندگان
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