کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1980593 | 1061867 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Near-full-length REV3L appears to be a scarce maternal factor in Xenopus laevis eggs that changes qualitatively in early embryonic development
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کلمات کلیدی
TLSORFNonidet P-40PAGENP-40PMSFLSSBSA - BSAcpd - CPDbovine serum albumin - آلبومین سرم گاوpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدImmunoprecipitation - تخریب ایمنیrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدDNA polymerase - دیانای پلی مراز، DNA پلیمرازEmbryonic development - رشد رویانtranslesion DNA synthesis - سنتز DNA ترجمه شدهtranslesion synthesis - سنتز ترجمهPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدopen reading frame - قاب خواندن بازRace - مسابقهVertebrate - مهره دارانpol - پل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
REV3 is the catalytic subunit of DNA polymerase ζ (pol ζ), which is responsible for the damage-induced mutagenesis that arises during error-prone translesion synthesis in eukaryotes. The related REV3L genes in human and mouse encode proteins of approximately 350 kDa, twice as large as yeast REV3, but full-length REV3L has not been identified in any vertebrate cell. We report that Xenopus laevis REV3L encodes a 352-kDa protein that has high overall amino acid sequence similarity to its mammalian counterparts, and, for the first time in a vertebrate species, we have detected putative REV3L polypeptides of 300 and 340 kDa in X. laevis oocytes. Only the 300-kDa form is stored in eggs, where its concentration of about 65 pM is much lower than those of other replication and repair proteins including the accessory pol ζ subunit REV7. In fertilized eggs, the levels of this polypeptide did not change until neurula; the larger 340-kDa form first appeared at stages after gastrula, suggesting a pattern of regulation during development. These observations indicate the existence of REV3L as a scarce protein, of approximately the full predicted size, whose level may impose severe constraints on the assembly of pol ζ in X. laevis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 9, Issue 1, 2 January 2010, Pages 90-95
Journal: DNA Repair - Volume 9, Issue 1, 2 January 2010, Pages 90-95
نویسندگان
Daichi Ogawara, Taketo Muroya, Kazumi Yamauchi, Taka-aki Iwamoto, Yoshihiko Yagi, Yoshihiro Yamashita, Shou Waga, Masahiro Akiyama, Hisaji Maki,