کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1981522 | 1061950 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Impaired DNA double strand break repair in cells from Nijmegen breakage syndrome patients
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DSBPSSeGFPMCsNHEJEYFPNBSMicrohomologySingle strand annealing - انلینگ رشته ایdouble strand break repair - تعمیر دو ردیفmultiple cloning site - سایت کلونینگ چندگانهNijmegen breakage syndrome - سندرم شکستن نیهمگنnon-homologous end-joining - عدم پیوستن به همولوگHomologous recombination - نوترکیبی همولوگenhanced yellow fluorescent protein - پروتئین فلورسنت زرد افزایش یافته استenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته است
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Impaired DNA double strand break repair in cells from Nijmegen breakage syndrome patients Impaired DNA double strand break repair in cells from Nijmegen breakage syndrome patients](/preview/png/1981522.png)
چکیده انگلیسی
Nijmegen breakage syndrome, caused by mutations in the NBS1 gene, is an autosomal recessive chromosomal instability disorder characterized by cancer predisposition. Cells isolated from Nijmegen breakage syndrome patients display increased levels of spontaneous chromosome aberrations and sensitivity to ionizing radiation. Here, we have investigated DNA double strand break repair pathways of homologous recombination, including single strand annealing, and non-homologous end-joining in Nijmegen breakage syndrome patient cells. We used recently developed GFP-YFP-based plasmid substrates to measure the efficiency of DNA double strand break repair. Both single strand annealing and non-homologous end-joining processes were markedly impaired in NBS1-deficient cells, and repair proficiency was restored upon re-introduction of full length NBS1 cDNA. Despite the observed defects in the repair efficiency, no apparent differences in homologous recombination or non-homologous end-joining effector proteins RAD51, KU70, KU86, or DNA-PKCS were observed. Furthermore, comparative analysis of junction sequences of plasmids recovered from NBS1-deficient and NBS1-complemented cells revealed increased dependence on microhomology-mediated end-joining DNA repair process in NBS1-complemented cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 5, Issue 2, 3 February 2006, Pages 251-257
Journal: DNA Repair - Volume 5, Issue 2, 3 February 2006, Pages 251-257
نویسندگان
Niall G. Howlett, Zorica Scuric, Alan D. D'Andrea, Robert H. Schiestl,