کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1981706 | 1539420 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Small RNA expression and deep sequencing analyses of the nucleolus reveal the presence of nucleolus-associated microRNAs
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کلمات کلیدی
qPCRrRNACrm1snoRNAActDLNARibosomal RNA - RNA Ribosomalshort interfering RNA - RNA تداخل کوتاهRNA polymerase I - RNA پلیمراز Ismall RNA - RNA کوچکsmall nucleolar RNA - RNA کوچک هسته ایsiRNA - siRNALocked Nucleic Acid - اسید نوکلئیک قفل شدهactinomycin D - اکتینومایسین DDeep sequencing - ترتیب عمیقpre - قبل ازNucleolus - هستکquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPol I - پل منprecursor - پیشگام
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Micro RNAs (miRNA) are non-coding RNAs expressed in the cytoplasm as their mature, 21-22-nucleotide short forms. More recently, mature miRNAs have also been detected in the nucleus, raising the possibility that their spatial distribution may be more complex than anticipated. Here we undertook comprehensive systematic analyses of miRNA distribution in several subcellular compartments of human cancer cells. In particular, we focused on the potential presence of miRNAs in the nucleolus, which contains an abundance of small non-coding RNAs. We employed two miRNA expression array platforms and small RNA deep sequencing of small RNAs isolated from cells, nuclei, cytoplasm and the nucleoli. We developed an assay to compare RNAs of isolated nucleoli before and after denaturation and used Northern hybridization to verify the presence of miRNAs in the subcellular compartments. Consistently, we found more than 10 miRNAs associated with the nucleolar preparations. Several miRNAs had greater relative abundance in the nucleolus compared to the other compartments. The nucleolar presence of miRNAs was independent of Dicer and the main activity of the nucleolus, RNA polymerase I transcription, but was dependent on CRM1 previously associated with nucleolar trafficking of small nucleolar RNAs. These results highlight the complexity of miRNA spatial arrangement and regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Open Bio - Volume 4, 2014, Pages 441-449
Journal: FEBS Open Bio - Volume 4, 2014, Pages 441-449
نویسندگان
Baoyan Bai, Hester Liu, Marikki Laiho,