کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1981843 1539422 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant
چکیده انگلیسی

Complementation for virulence of a non-polar virB5 mutant in Brucella suis 1330 was not possible using a pBBR-based plasmid but was with low copy vector pGL10. Presence of the pBBR-based replicon in wildtype B. suis had a dominant negative effect, leading to complete attenuation in J774 macrophages. This was due to pleiotropic effects on VirB protein expression due to multiple copies of the virB promoter region and over expression of VirB5. Functional complementation of mutants in individual components of multiprotein complexes such as bacterial secretion systems, are often problematic; this study highlights the importance of using a low copy vector.

▸ A Brucella suis virB5 mutant is attenuated in J774 macrophages. ▸ VirB5 over expression and/or multiple virB promoter copies attenuate WT B. suis. ▸ VirB5 over expression and/or multiple virB promoter copies affect other VirB proteins. ▸ Expressing virB5 from a low copy number plasmid fully complements a virB5 mutant.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Open Bio - Volume 2, 2012, Pages 71–75
نویسندگان
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