Intracellular metabolic changes of Clostridium acetobutylicum and promotion to butanol tolerance during biobutanol fermentation

GCâ¿¿MS-based metabolomics approach is effective for elucidating underlying response of Clostridium acetobutylicum to butanol stress. PLS-DA revealed a group classification and pairwise discrimination, and 27 metabolites with VIP value greater than 1 were extracted by PLS-DA. The metabolic relevance of these compounds in the response of C. acetobutylicum to butanol stress was discussed.