کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1983644 | 1539926 | 2011 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A calcium-insensitive attenuated nitrosative stress response contributes significantly in the radioresistance of Sf9 insect cells
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کلمات کلیدی
L-NMMADCFDANOSDAF2′,7′-dichlorofluorescein diacetate - 2 '، 7'-dichlorofluorescein diacetate4,5-diaminofluorescein diacetate - 4،5-دی آمین فلوئورسین دی سکتهionizing radiation - تابش یوننده یا پرتوهای یونیزانApoptosis - خزان یاختهایelectron transport chain - زنجیره انتقال الکترونCAM - ساخت به کمک کامپیوترNitric oxide synthase (NOS) - نیتریک اکساید سنتاز (NOS)Nitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازETc - و غیرهCalmodulin - کالمودولینCalcium - کلسیمReactive oxygen species (ROS) - گونه های اکسیژن واکنشی (ROS)
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A calcium-insensitive attenuated nitrosative stress response contributes significantly in the radioresistance of Sf9 insect cells A calcium-insensitive attenuated nitrosative stress response contributes significantly in the radioresistance of Sf9 insect cells](/preview/png/1983644.png)
چکیده انگلیسی
Lepidopteran insects/insect cells display 50-100 times higher radioresistance than humans, and are evolutionarily closest to mammals amongst all radioresistant organisms known. Compared to mammalian cells, Lepidopteran cells (TN-368, Sf9) display more efficient antioxidant system and DNA repair and suffer considerably less radiation-induced DNA/cytogenetic damage and apoptosis. Recent studies indicate that a considerably lower radiation-induced oxidative stress may significantly reduce macromolecular damage in Lepidopteran cells. Since nitrosative stress contributes in radiation-induced cellular damage, we investigated its nature in the γ-irradiated Sf9 cells (derived from Spodoptera frugiperda; order Lepidoptera; family Noctuidae) and compared with BMG-1 human cell line having significant NOS expression. Radiation induced considerably less ROS/RNS in Sf9 cells, which remained unchanged on treatment with NOS inhibitor l-NMMA. Surprisingly, growth of Sf9 cultures or irradiation could not induce NO or its metabolites, indicating negligible basal/radiation-induced NOS activity that remained unchanged even after supplementation with arginine. Cytosolic calcium release following high-dose (1000-2000 Gy at 61.1 cGy sâ1) γ-irradiation or H2O2 (250 μM) treatment also failed to generate NO in Sf9 cells having high constitutive levels of calmodulin, whereas BMG-1 cells displayed considerable calcium-dependent NO generation even following 10 Gy dose. These results strongly imply the lack of calcium-mediated NOS activity in Sf9 cells. Addition of exogenous NO from GSH-NO caused considerable increase in radiation-induced apoptosis, indicating significant contribution of constitutively attenuated nitrosative stress response into the radioresistance of Lepidopteran cells. Our study demonstrates for the first time that a calcium-insensitive, attenuated nitrosative stress response may contribute significantly in the unusual radioresistance displayed by Lepidopteran insect cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 43, Issue 9, September 2011, Pages 1340-1353
Journal: The International Journal of Biochemistry & Cell Biology - Volume 43, Issue 9, September 2011, Pages 1340-1353
نویسندگان
Shubhankar Suman, Rakesh Kumar Seth, Sudhir Chandna,