کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1990992 1540734 2008 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Loss of PPARγ in immune cells impairs the ability of abscisic acid to improve insulin sensitivity by suppressing monocyte chemoattractant protein-1 expression and macrophage infiltration into white adipose tissue
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Loss of PPARγ in immune cells impairs the ability of abscisic acid to improve insulin sensitivity by suppressing monocyte chemoattractant protein-1 expression and macrophage infiltration into white adipose tissue
چکیده انگلیسی

Abscisic acid (ABA) is a natural phytohormone and peroxisome proliferator-activated receptor γ (PPARγ) agonist that significantly improves insulin sensitivity in db/db mice. Although it has become clear that obesity is associated with macrophage infiltration into white adipose tissue (WAT), the phenotype of adipose tissue macrophages (ATMs) and the mechanisms by which insulin-sensitizing compounds modulate their infiltration remain unknown. We used a loss-of-function approach to investigate whether ABA ameliorates insulin resistance through a mechanism dependent on immune cell PPARγ. We characterized two phenotypically distinct ATM subsets in db/db mice based on their surface expression of F4/80. F4/80hi ATMs were more abundant and expressed greater concentrations of chemokine receptor (CCR) 2 and CCR5 when compared to F4/80lo ATMs. ABA significantly decreased CCR2+ F4/80hi infiltration into WAT and suppressed monocyte chemoattractant protein-1 (MCP-1) expression in WAT and plasma. Furthermore, the deficiency of PPARγ in immune cells, including macrophages, impaired the ability of ABA to suppress the infiltration of F4/80hi ATMs into WAT, to repress WAT MCP-1 expression and to improve glucose tolerance. We provide molecular evidence in vivo demonstrating that ABA improves insulin sensitivity and obesity-related inflammation by inhibiting MCP-1 expression and F4/80hi ATM infiltration through a PPARγ-dependent mechanism.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 19, Issue 4, April 2008, Pages 216–228
نویسندگان
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