کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1991082 | 1540746 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phospholipase D as a potential target for the antiproliferative effects of polyunsaturated fatty acids in rat thymocytes
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کلمات کلیدی
Concanavalin A7-AAD7-aminoactinomycinConAFCSHSA14:0 - 14: 03-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyl tetrazolium bromideMTT - MTThuman serum albumin - آلبومین سرم انسانیEicosapentaenoic acid - اسید ایکوزاپنتانوئیکdocosapentaenoic acid - اسید داکوزاپنتانوئیکdocosahexaenoic acid - اسید داکوزاگزوائونیکphosphatidic acid - اسید فسفاتیدیکLinoleic acid - اسید لینولئیکMyristic acid - اسید مریستیکFatty acid - اسید چربPolyunsaturated fatty acid - اسید چرب غیر اشباعPUFA - اسید چرب چند غیراشباعfetal calf serum - سرم گوساله جنینphosphatidylcholine - فسفاتیدیل کولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The effects of various saturated and unsaturated fatty acids (FAs) on the proliferative response and phospholipase D (PLD) activity of rat thymocytes were investigated. When added to culture medium as complexes with albumin, all the FAs tested, except stearic acid, inhibited the ConA-induced thymocyte proliferation, eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids being the most inhibitory. Apart from 22:6n-3 which slightly increased the percentage of late apoptotic and necrotic thymocytes in the presence of mitogen, none of the FAs induced significant apoptosis or necrosis. A short 2-h preincubation of rat thymocytes in the presence of FA-albumin complexes was sufficient to induce a significant enrichment of cell phospholipids with each FA and to stimulate thymocyte PLD activity. However, 20:5n-3 was inactive despite a large enrichment in phospholipids. Furthermore, the PLD activity of activated thymocytes was negatively correlated to the proliferative response, with the exception of 20:5n-3-supplemented cells. These results support further our current hypothesis that PLD activity conveys antiproliferative signals in lymphoid cells, and suggest that 20:5n-3 inhibits thymocyte proliferation by a particular mechanism unrelated to that of the other FAs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 18, Issue 4, April 2007, Pages 228-235
Journal: The Journal of Nutritional Biochemistry - Volume 18, Issue 4, April 2007, Pages 228-235
نویسندگان
Amal Benzaria, Nadia Meskini, Madeleine Dubois, Georges Némoz, Michel Lagarde, Annie-France Prigent,