کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1994016 1064725 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Apoptosis-associated mitochondrial outer membrane permeabilization assays
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Apoptosis-associated mitochondrial outer membrane permeabilization assays
چکیده انگلیسی

Following most cell death signals, pro-apoptotic Bcl-2 members as Bax and Bak are activated and oligomerize into the mitochondria outer membrane, triggering its permeabilization and release into the cytosol of soluble apoptogenic factors such as cytochrome c involved in caspase activation. Thus, in many studies focused on apoptosis, cytochrome c release within cells is frequently examined to assess Bax/Bak activation and mitochondrial outer membrane permeabilization. In addition, cytochrome c release can also be investigated in vitro in functional mitochondria that have been isolated from cultured cells, offering a number of advantages. Here, protocols for measuring cytochrome c release from intact cells as well as from isolated mitochondria is detailed. Finally, assays to investigate Bax/Bak activation and olimerization are also presented.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 44, Issue 3, March 2008, Pages 229–234
نویسندگان
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