کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2006977 | 1066360 | 2008 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Predicted versus expressed adipokinetic hormones, and other small peptides from the corpus cardiacum-corpus allatum: A case study with beetles and moths
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کلمات کلیدی
TOFManduca sextaTenebrio molitorPBANNVPMelolontha melolonthaMamestra brassicaeAnopheles gambiaePeriplaneta americanaSpodoptera littoralisSpodoptera frugiperdaPyrokininTFAPSDHeliothis zeatBMELeucophaea maderaeLacanobia oleraceaESICAP2bCIDPyrrhocoris apterusPyrokininsMyosuppressinMS/MS - MS / MSSchistocerca gregaria - Schistocerca گرگاریاAKH - آهCollision-induced dissociation - اختلاف ناشی از برخوردTrifluoroacetic acid - اسید Trifluoroaceticcorpora allata - بدن آورده شده استmatrix-assisted laser desorption/ionization - جذب / یونیزاسیون لیزر ماتریس کمک می کندInsects - حشراتRetention time - زمان بازداریTime-of-Flight - زمان پروازMass spectrometry - طیف سنجی جرمیTandem mass spectrometry - طیف سنجی جرمی پشت سر هم یا متوالیPost-source decay - فروپاشی پست منبعcorpora cardiaca - قلب corporaMALDI - مالدیMALDI-TOF MS - مالدی توف MSLocusta migratoria - مهاجرت لاکتوسMyotropin - میوتروپینNeuropeptides - نوروپپتیدهاAdipokinetic hormone - هورمون آدپیوکینتیکdiapause hormone - هورمون دیافراگمHypertrehalosemic hormone - هورمون هیپرترهالوزمیکTribolium castaneum - کاکتوس TriboliumBombyx mori - کرم ابریشمliquid chromatography - کروماتوگرافی مایعelectrospray ionization - یونیزاسیون الکترو اسپری
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Predicted versus expressed adipokinetic hormones, and other small peptides from the corpus cardiacum-corpus allatum: A case study with beetles and moths Predicted versus expressed adipokinetic hormones, and other small peptides from the corpus cardiacum-corpus allatum: A case study with beetles and moths](/preview/png/2006977.png)
چکیده انگلیسی
This mass spectrometric study confines itself to peptide masses in the range of 500-1500 Da. Adipokinetic hormones (AKHs) that are predicted from the genome of the red flour beetle, Tribolium castaneum, and the silk moth, Bombyx mori, are shown to exist as expressed peptides in the corpora cardiaca (CC) of the respective species as evidenced by various mass spectrometric methods. Additionally, some related species were included in this study, such as the tenebrionid beetles Tribolium brevicornis and Tenebrio molitor, as well as the moths Spodoptera frugiperda, Spodoptera littoralis, Mamestra brassicae and Lacanobia oleracea, to investigate whether AKH peptides are structurally conserved in the same genus or family. Interestingly, the AKH peptide of T. brevicornis is identical to that of T. molitor but not to the ones of its close relative T. castaneum. Moreover, other peptides in T. brevicornis, such as various FXPRL amides (=pyrokinins), also match the complement in T. molitor but differ from those in T. castaneum. All the CC of beetles lacked the signal for the mass of the peptide corazonin. All moths have the nonapeptide Manse-AKH expressed in their CC. In addition, whereas the silk moth has the decapeptide Bommo-AKH as a second peptide, all other moths (all noctuids) express the decapeptide Helze-HrTH. In M. brassicae and L. oleracea a novel amidated Gly-extended Manse-AKH is found as a possible third AKH. The noctuid moth species also all express the same FLRF amide-I, corazonin, and a group-specific isoform of a γ-PGN-(=γ-SGNP) peptide. In L. oleracea, however, the latter peptide has a novel sequence which is reported for the first time, and the peptide is code-named Lacol-PK.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 29, Issue 7, July 2008, Pages 1124-1139
Journal: Peptides - Volume 29, Issue 7, July 2008, Pages 1124-1139
نویسندگان
Gerd Gäde, Heather G. Marco, Petr Å imek, Neil Audsley, Kevin D. Clark, Robert J. Weaver,