کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2014812 | 1541941 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of flavonoid biosynthesis genes and accumulation of flavonoid in wheat leaves in response to drought stress
ترجمه فارسی عنوان
بیان ژن های بیوسنتز فلاونوئید و تجمع فلاونوئید در برگ های گندم در پاسخ به تنش خشکی
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کلمات کلیدی
FNSFLSCHICHSdihydroflavonol-4-reductaseqRT-PCRF3HDFRROS - ROSChinese spring - بهار چینیGene expression - بیان ژنDrought - خشکسالیANS - سالchalcone synthase - سنتاز چالکونflavone synthase - فلاون سنتازflavonol synthase - فلاونول سنتازflavanone 3-hydroxylase - فلاونون 3-هیدروکسیلازquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراchalcone isomerase - کلومون ایزومرازWheat - گندمReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
چکیده انگلیسی
Flavonoids are the low molecular weight polyphenolic secondary metabolic compounds, and have various functions in growth, development, reproduction, and stress defense. However, little is known about the roles of the key enzymes in the flavonoids biosynthesis pathway in response to drought stress in winter wheat. Here, we investigated the expression pattern of flavonoids biosynthesis genes and accumulation of flavonoids in wheat leaves under drought stress. Quantitative real-time PCR analysis showed that there were a rapid increase in expression levels of TaCHS, TaCHI, TaF3H, TaFNS, TaFLS, TaDFR, and TaANS under drought stress in two wheat cultivars Aikang 58 (AK) and Chinese Spring (CS). The cultivar CS exhibited higher genes expression levels of TaCHS, TaCHI, TaF3H, TaFLS, TaDFR, and TaANS, and the cultivar AK showed a higher expression level of TaFNS gene during drought treatment. The increase rates of genes expression were superior in AK compared to CS. Total phenolics content, total flavonoids content, anthocyanin content, and schaftoside content in wheat leaves were enhanced during drought treatment and cultivar CS had a relative higher accumulation. These results suggest that the flavonoids pathway genes expression and accumulation of flavonoids compounds may be closely related to drought tolerant in wheat. Further, flavonoids response mechanism may be different between wheat cultivars.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 80, July 2014, Pages 60-66
Journal: Plant Physiology and Biochemistry - Volume 80, July 2014, Pages 60-66
نویسندگان
Dongyun Ma, Dexiang Sun, Chenyang Wang, Yaoguang Li, Tiancai Guo,