Characterization of two novel subtilases from common bean (Phaseolus vulgaris L.) and their responses to drought

► A novel subtilase affected by drought and senescence was isolated from common bean. ► It was characterized at the gene, mRNA transcript and protein levels. ► Its activity in mature and young leaves increased on withdrawal of water. ► A second novel subtilase from common bean was identified at the gene level. ► Drought had no effect on the expression of the transcript of either subtilase.