کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2015470 1541975 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Partial purification and characterization of a copper-induced anionic peroxidase of sunflower roots
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Partial purification and characterization of a copper-induced anionic peroxidase of sunflower roots
چکیده انگلیسی

Treatment of 14-day-old sunflower seedlings with a toxic amount of copper (50 μM of CuSO4) during 5 days caused significant increase in peroxidase activity in roots. Qualitative analysis of soluble proteins using native anionic PAGE followed by detection of peroxidase activity with guaïacol as electron donor in the presence of H2O2 revealed five stimulated peroxidases, named A1, A2, A3, A4, and A5. These peroxidases had differential behavior during the period of treatment. A1, A2, A3 and A4 were stimulated in the first period of stress, but rapidly suppressed at 72 h. A5 showed a progressive stimulation which was even increased at 120 h. A1 was partially purified, identified using liquid chromatography coupled to mass spectrometry (LC-MS/MS), and characterized. Effects of pH and temperature on its activity were determined with guaïacol as electron donor. Optima were obtained at pH 8 and at 40 °C. Analysis of substrate specificity showed that A1 was active on coniferyl alcohol but not on IAA. Enzymatic activity was inhibited by a high concentration of H2O2.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 46, Issues 8–9, August–September 2008, Pages 760–767
نویسندگان
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