کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2016719 | 1067683 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transgenic ipt tobacco overproducing cytokinins overaccumulates phenolic compounds during in vitro growth
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کلمات کلیدی
PODPssuSPODGPODPathogenesis related proteinsIPTIAADAB3,3′-diaminobenzidine - 3،3'-diaminobenzidineSalicylic acid - اسید سالیسیلیکPhenolic acids - اسیدهای فنولیکsyringaldazine peroxidase - سرینگالدازین پراکسیدازCytokinins - سیتوکینین هاPeroxidase - پراکسیدازPeroxidases - پراکسیدازهاPR proteins - پروتئین های PRIn vitro cultivation - کشت در محیط کشتguaiacol peroxidase - گوایاکول پراکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We present evidence that overproduction of endogenous cytokinins (CK) caused stress response in non-rooting Pssu-ipt transgenic tobacco (Nicotiana tabacum L.) grown in vitro. It was demonstrated by overaccumulation of phenolic compounds, synthesis of pathogenesis related proteins (PR proteins), and increase in peroxidase (POD) activities. Immunolocalization of zeatin and also PR-1b protein on leaf cryo-sections proved their accumulation in all mesophyll cells of transgenic tobacco contrary to control non-transgenic plants. Intensive blue autofluorescence of phenolic compounds induced by UV in cross-sections of leaf midrib showed enhanced contents of phenolics in transgenic tobacco compared with controls, nevertheless, no significant difference between both plant types was found in leaf total lignin content. Transgenic plantlets exhibited higher peroxidase activities of both soluble and ionically bound fractions compared with controls. HPLC analysis of phenolic acids confirmed the increase in all phenolic acids in transgenic tobacco except for salicylic acid (SA). The effect of high phenolic content on rooting of transgenic tobacco is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 44, Issue 10, October 2006, Pages 526-534
Journal: Plant Physiology and Biochemistry - Volume 44, Issue 10, October 2006, Pages 526-534
نویسندگان
Renáta Schnablová, Helena Synková, Anna ViÄánková, Lenka Burketová, Josef Eder, Milena Cvikrová,