کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2016934 | 1542041 | 2016 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Overexpression of wheat ubiquitin gene, Ta-Ub2, improves abiotic stress tolerance of Brachypodium distachyon
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کلمات کلیدی
Brachypodium distachyonPODCATCBFRWCAPXSWCROS - ROSEnzymatic antioxidants - آنتی اکسیدان های آنزیمیAbiotic stress tolerance - تحمل استبدادیGrowth and development - رشد و توسعهSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازC-repeat binding factor - عامل اتصال C تکرارTranscription factors - عوامل رونویسیSoil water content - محتوای آب خاکRelative water content - محتوای آب نسبیwild type - نوع وحشیPeroxidase - پراکسیدازascorbate peroxidase - پراکسیداز آسکورباتیpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول Catalase - کاتالازReactive oxygen species - گونههای فعال اکسیژنUPS - یو پی اسUbiquitin - یوبیکویتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ubiquitination plays an important role in regulating plant's development and adaptability to abiotic stress. To investigate the possible functions of a wheat monoubiquitin gene Ta-Ub2 in abiotic stress in monocot and compare it with that in dicot, we generated transgenic Brachypodium plants overexpressing Ta-Ub2 under the control of CaMV35s and stress-inducible RD29A promoters. The constitutive expression of Ta-Ub2 displayed slight growth inhibition in the growth of transgenic Brachypodium distachyon under the control conditions. However, this inhibition was minimized by expression of Ta-Ub2 under the control of stress-inducible RD29A promoter. Compared with WT, the transgenic plants preserved more water and showed higher enzymatic antioxidants under drought stress, which might be related to the change in the expression of some antioxidant genes. The expression of C-repeat binding factors transcription factor genes in the transgenic B. distachyon lines were upregulated under water stress. Salt and cold tolerances of transgenic B. distachyon were also improved. Although the phenotypic changes in the transgenic plants were different, overexpression of Ta-Ub2 improved the abiotic stress tolerance in both dicot and monocot plants. The improvement in Ta-Ub2 transgenic plants in abiotic stress tolerance might be, at least partly, through regulating the gene expression and increasing the enzymatic antioxidants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 248, July 2016, Pages 102-115
Journal: Plant Science - Volume 248, July 2016, Pages 102-115
نویسندگان
Hanhan Kang, Meng Zhang, Shumei Zhou, Qifang Guo, Fengjuan Chen, Jiajie Wu, Wei Wang,