کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2017029 | 1542053 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rubisco accumulation is important for the greening of the fln2-4 mutant in Arabidopsis
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کلمات کلیدی
Chloroplast transit peptideSUCCTPRuBisCOEtBrPALPEPribulose-1,5-bisphosphate carboxylase/oxygenase - Ribulose-1،5-Bisphosphate کربوکسیلاز / OxygenaseArabidopsis - آرابیدوپسیس یا رشادیethidium bromide - اتیدیوم برومایدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتFru - زنSucrose - ساکارزFructose - فروکتوز Palatinose - پالتینوزGlu - گلوGlucose - گلوکز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Rubisco accumulation is important for the greening of the fln2-4 mutant in Arabidopsis Rubisco accumulation is important for the greening of the fln2-4 mutant in Arabidopsis](/preview/png/2017029.png)
چکیده انگلیسی
The fructokinase-like protein2 (FLN2) is a component of the PEP complex. FLN2 knockout mutants displayed a delayed greening phenotype on sucrose-containing medium. Our previous work indicated that partial PEP activity is essential for its greening phenotype. In this study, we further report that sufficient Rubisco accumulation is critical for fln2-4 greening. Sugar serves many important functions, such as an energy source and signaling molecule. Through pharmacological experiments using a sugar analog and sugar signaling inhibitor, we demonstrate that sugar serves as energy to support the fln2-4 greening. Seed-reserve and photosynthetic CO2-fixation are the primary energy sources for early seedling growth. No obvious differences were observed in the seed-reserve of the wild-type and fln2-4 by comparing their seed size and dark-germination, indicating that the defective carbon fixation may account for the energy deficit in fln2-4 during its early seedling growth. The Rubisco content was low in fln2-4, but it rapidly accumulated during the greening of fln2-4. Expression of a nuclear-encoded rbcL gene facilitates Rubisco accumulation and partially complements the mutant defects. These results suggest that the Rubisco accumulation is critical for fln2-4 greening. In summary, the rapid Rubisco accumulation that depends on sufficient PEP activity is important for normal seedling growth.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 236, July 2015, Pages 185-194
Journal: Plant Science - Volume 236, July 2015, Pages 185-194
نویسندگان
Chao Huang, Qing-Bo Yu, Xin-Bo Yuan, Zi-Ran Li, Jing Wang, Lin-Shan Ye, Ling Xu, Zhong-Nan Yang,