Positive effects of temperature and growth conditions on enzymatic and antioxidant status in lettuce plants

The contents of two bioactive compounds (polyphenols and flavonoids) and their antioxidant and enzyme activities were determined in the leaves of six lettuce (Latuca sativa L.) cultivars subjected to 4 different day/night temperatures for 6 weeks.The total polyphenol and anthocyanin contents and the corresponding antioxidant activities were the highest at 13/10 °C and 20/13 °C, followed by 25/20 °C and 30/25 °C. The enzymatic activities of polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) were also the highest at low day/night temperatures, but the peroxidase (POD) activity was decreased at low day/night temperatures and increased at high day/night temperatures.The most significant positive correlation existed between anthocyanin content and PPO activity, total polyphenols and their antioxidant activities. The results showed that at relatively low temperatures, lettuce plants have a high antioxidant and enzymatic status. These results provide additional information for the lettuce growers.

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► Anthocyanins, polyphenols, antioxidants, polyphenol oxidase, peroxidase, phenylalanine ammonia-lyase.
► Leaves of six lettuce (Latuca sativa L.) cultivars.
► 4 different day/night temperatures for 6 weeks.
► Positive correlation between anthocyanin and polyphenol contents and polyphenol oxidase activity.
► Various day/night temperatures affect the anthocyanin content and antioxidant and enzyme activities.