کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2019433 | 1542208 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of CYP 4A Ï-hydroxylase and formation of 20-hydroxyeicosatetreanoic acid (20-HETE) in cultured rat brain astrocytes
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کلمات کلیدی
mGluRLC–ESI–MSHET001620-HETEKCanpoEETsSAHCBFGFAP20-Hydroxyeicosatetraenoic acid - 20-Hydroxyeicosatetraenoic اسیدLC–MS - LC-MSAstrocyte - آستروسیتArachidonic acid - اسید آراشیدونیکepoxyeicosatrienoic acids - اسید اپوکسی اسیاتریتروئینcerebral blood flow - جریان خون مغزیSubarachnoid hemorrhage - خونریزی زیر عنکبوتیهliquid chromatography–mass spectroscopy - طیف سنجی جرم کروماتوگرافی مایعneurovascular unit - واحد عصبی عضلانیGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالCerebral circulation - گردش خون مغزیMetabotropic glutamate receptor - گیرنده گلوتامات متابوتروپیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Astrocytes secrete vasodilator and vasoconstrictor factors via end feet processes, altering blood flow to meet neuronal metabolic demand. Compared to what is known about the ability of astrocytes to release factors that dilate local cerebral vasculature, very little is known regarding the source and identity of astrocyte derived constricting factors. The present study investigated if astrocytes express CYP 4A Ï-hydroxylase and metabolize arachidonic acid (AA) to 20-hydroxyeicotetraenoic acid (20-HETE) that regulates KCa channel activity in astrocytes and cerebral arterial myocyte contractility. Here we report that cultured astrocytes express CYP 4A2/3 Ï-hydroxylase mRNA and CYP 4A protein and produce 20-HETE and the CYP epoxygenase metabolites epoxyeicosatrienoic acids (EETs) when incubated with AA. The production of 20-HETE and EETs was enhanced following stimulation of metabotropic glutamate receptors (mGluR) on the astrocytes. Exogenous application of 20-HETE attenuated, whereas inhibition of 20-HETE production with HET-0016 increased the open state probabilities (NPo) of 71Â pS and 161Â pS KCa single-channel currents recorded from astrocytes. Exposure of isolated cerebral arterial myocytes to conditioned media from cultured astrocytes caused shortening of the length of freshly isolated cerebral arterial myocytes that was not evident following inhibition of astrocyte 20-HETE synthesis and action. These findings suggest that astrocytes not only release vasodilator EETs in response to mGluR stimulation but also synthetize and release the cerebral arterial myocyte constrictor 20-HETE that also functions as an endogenous inhibitor of the activity of two types of KCa channel currents found in astrocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins & Other Lipid Mediators - Volume 124, July 2016, Pages 16-26
Journal: Prostaglandins & Other Lipid Mediators - Volume 124, July 2016, Pages 16-26
نویسندگان
Debebe Gebremedhin, David X. Zhang, Koryn A. Carver, Nicole Rau, Kevin R. Rarick, Richard J. Roman, David R. Harder,