کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2057547 1075892 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular cloning and tissue-specific expression of two cDNAs encoding polyketide synthases from Hypericum perforatum
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Molecular cloning and tissue-specific expression of two cDNAs encoding polyketide synthases from Hypericum perforatum
چکیده انگلیسی

SummaryTwo previously uncharacterized cDNAs encoding for polyketide synthases (PKSs), designated as HpPKS1 and HpPKS2, were isolated from Hypericum perforatum. The full-length HpPKS1 was 1573 bp containing an open reading frame (ORF) of 1161 bp encoding for a 386 amino acid protein. The full-length cDNA of HpPKS2 was 1559 bp with an ORF of 1182 bp encoding for a 393 amino acid protein. The highly conserved catalytic amino acid residues common to plant-specific PKSs were preserved in both genes. HpPKS1 and HpPKS2 exhibited distinct tissue-specific expression patterns in H. perforatum. The HpPKS1 expression was highest in flower buds and lowest in root tissues. The expression of HpPKS2 was found to be high in flower buds and leaf margins and low in leaf interior parts, stems and roots. The expression of the HpPKS1 was found to correlate with the concentrations of hyperforin and adhyperforin while the expression of HpPKS2 showed correlation with the concentrations of hypericins and pseudohypericins in H. perforatum tissues.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 165, Issue 10, 7 July 2008, Pages 1079–1086
نویسندگان
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