کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2058391 1076226 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular cloning of calnexin and calreticulin in the Pacific oyster Crassostrea gigas and its expression in response to air exposure
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات علوم زمین و سیاره ای (عمومی)
پیش نمایش صفحه اول مقاله
Molecular cloning of calnexin and calreticulin in the Pacific oyster Crassostrea gigas and its expression in response to air exposure
چکیده انگلیسی

Calnexin (CNX) and calreticulin (CRT) are endoplasmic reticulum (ER) chaperones. CNX is a type I transmembrane protein and CRT is a soluble CNX homologue. In the ER, CNX and CRT are important for Ca2+ homeostasis and protein maturation. Here, we describe the full-length cDNA of the first mollusk CNX (cgCNX) and a second mollusk CRT (cgCRT) from the oyster Crassostrea gigas. CgCNX, containing 3255 bp, was composed of a 1764 bp open reading frame (ORF) that encodes a 588-amino acid protein. CgCRT, containing 1727 bp, was composed of a 1242 bp ORF that encodes a 414-amino acid protein. CgCNX and cgCRT contains an N-terminal 21- and 16-amino acid sequence, respectively, which is characteristic of a signal sequence. At the C-terminus, cgCRT also contains the KDEL (-Lys-Asp-Glu-Leu) peptide motif suggesting that cgCRT localizes in the ER. Northern blot analysis showed that both cgCNX and cgCRT mRNAs are induced by air exposure. The expression patterns of cgCNX mRNA differed from those of cgCRT during air exposure. This suggests that these two molecular chaperones have different roles in the response to air exposure.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Genomics - Volume 3, Issue 1, March 2010, Pages 19–27
نویسندگان
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