کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2078867 | 1545057 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High Output of a Trametes Laccase in Pichia pastoris and Characterization of Recombinant Enzymes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High Output of a Trametes Laccase in Pichia pastoris and Characterization of Recombinant Enzymes High Output of a Trametes Laccase in Pichia pastoris and Characterization of Recombinant Enzymes](/preview/png/2078867.png)
چکیده انگلیسی
A laccase gene (lacD) from the basidiomycete Trametes sp. 420 was heterologously expressed in Pichia pastoris in two ways, resulting in two recombinant enzymes of rLacDx with native N-terminus and rLacDe with eight additional amino acid residues at N-terminus. The yields of rLacDx and rLacDe in shaken-flask cultures after an 18-day growth were 1.21Ã105 u/L and 7.38Ã104 u/L, respectively, as determined with 2,2â²-azinobis (3-ethylbenzothia-zoline- 6-sulfonic acid) (ABTS) as substrate. The yield of rLacDx was further increased to 2.39Ã105 U/l under high-density fermentation while the production process was decreased to 7.5 days. In addition, rLacDx and rLacDe exhibited similar enzymatic characters in oxidizing substrate guaiacol, and were stable at 50°C and at a pH range from 3 to 10. However, the specific activity of rLacDx (1761 u/mg) for ABTS was higher than that of rLacDe (1122 u/mg), and the apparent Km value of rLacDx (427 μmol/L) was less than that of rLacDe (604 μmol/L).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 23, Issue 6, November 2007, Pages 1055-1059
Journal: Chinese Journal of Biotechnology - Volume 23, Issue 6, November 2007, Pages 1055-1059
نویسندگان
ZHOU Hong-Min, HONG Yu-Zhi, XIAO Ya-Zhong, CUI Teng-Jiao, WANG Xiao-Tang, PU Chun-Lei,