کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2129978 1086515 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In-depth evaluation of commercially available human vascular smooth muscle cells phenotype: Implications for vascular tissue engineering
ترجمه فارسی عنوان
بررسی عمیق فنوتیپ سلول های عضلانی انسان در معرض عموم: پیامدهای مهندسی بافت عروقی
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
چکیده انگلیسی


• Ability of human smooth muscle cells to alter phenotype in culture is evaluated.
• Examined the effect of passaging human smooth muscle cells on phenotype.
• Phenotype is assessed based on morphology, proliferation, markers, and migration.
• Multi-resolution assessment methodology, single-cell and cell-population.
• Lower and higher passages than P7 adopted a contractile and synthetic phenotype respectively.

In vitro research on vascular tissue engineering has extensively used isolated primary human or animal smooth muscle cells (SMC). Research programs that lack such facilities tend towards commercially available primary cells sources. Here, we aim to evaluate the capacity of commercially available human SMC to maintain their contractile phenotype, and determine if dedifferentiation towards the synthetic phenotype occurs in response to conventional cell culture and passaging without any external biochemical or mechanical stimuli. Lower passage SMC adopted a contractile phenotype marked by a relatively slower proliferation rate, higher expression of proteins of the contractile apparatus and smoothelin, elongated morphology, and reduced deposition of collagen types I and III. As the passage number increased, migratory capacity was enhanced, average cell speed, total distance and net distance travelled increased up to passage 8. Through the various assays, corroborative evidence pinpoints SMC at passage 7 as the transition point between the contractile and synthetic phenotypes, while passage 8 distinctly and consistently exhibited characteristics of synthetic phenotype. This knowledge is particularly useful in selecting SMC of appropriate passage number for the target vascular tissue engineering application, for example, a homeostatic vascular graft for blood vessel replacement versus recreating atherosclerotic blood vessel model in vitro.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 343, Issue 2, 1 May 2016, Pages 168–176
نویسندگان
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