کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2130486 | 1086576 | 2012 | 8 صفحه PDF | دانلود رایگان |

It has long been known that core body temperature declines with age, with temperatures of 35.5 °C or below common in the elderly. However, the effects of temperature reduction on bone cell function and skeletal homeostasis have been little studied. We investigated the effects of mild hypothermia (35.5 °C) and severe hypothermia (34 °C) on bone-forming osteoblasts, and bone-resorbing osteoclasts. Formation of ‘trabecular’ bone structures by rat calvarial osteoblasts was reduced by 75% at 35.5 °C and by 95% at 34 °C after 14–16 days culture, compared to 37 °C. In addition to reductions in osteoblast cell number, expression of mRNAs for Runx2, alkaline phosphatase, osteocalcin and type I collagen were also down-regulated in hypothermia. In contrast, formation of osteoclasts in mononuclear cell cultures derived from mouse marrow, showed a 1.5 to 2-fold stimulation in hypothermia; resorption pit formation was similarly increased. Taken together, these data show that hypothermia exerts reciprocal effects on bone cell function by retarding osteoblast differentiation and bone formation, whilst increasing osteoclastogenesis and thus resorption. These results suggest the possibility that hypothermia in the elderly could potentially have a direct, negative impact on bone metabolism.
► Chronic hypothermia is common in elderly humans but its effects on bone are unclear.
► We studied the effects of hypothermia on bone cell function in primary culture.
► Bone formation by osteoblasts was retarded by 75% at 35.5 °C and 95% at 34 °C.
► Formation of osteoclasts and resorption pits increased up to 90% at 34 °C–35.5 °C.
► This suggests that mild hypothermia could have a negative impact on bone turnover.
Journal: Experimental Cell Research - Volume 318, Issue 17, 15 October 2012, Pages 2237–2244