Melanogenesis stimulation in B16-F10 melanoma cells induces cell cycle alterations, increased ROS levels and a differential expression of proteins as revealed by proteomic analysis

► Melanogenesis stimulation by l-tyrosine+NH4Cl in B16-F10 melanoma cells increases ROS levels. ► Melanogenesis inhibits cell proliferation, and induced cell cycle arrest in the G1 phase. ► Proteomic analysis showed alterations in proteins of the cell cycle and glucose metabolism. ► RT-qPCR analysis confirmed alterations of metabolic targets after melanogenesis stimulation.