کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2130651 | 1086589 | 2012 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tissue protection and endothelial cell signaling by 20-HETE analogs in intact ex vivo lung slices
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کلمات کلیدی
DMEMFITCDcfdichlorofluoresceinPECAM20-HETEBPAECPEG-SOD20-Hydroxyeicosatetraenoic acid - 20-Hydroxyeicosatetraenoic اسیدdihydroethidium - دی هیدروتیدیمEndothelial cell - سلول های اندوتلیالfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتlow density lipoprotein - لیپوپروتئین چگالی کمLDL - لیپوپروتئین کم چگالی(کلسترول بد)platelet endothelial cell adhesion molecule - مولکول چسبندگی سلول اندوتلیال پلاکتDHE - و
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The capacity to follow cell type-specific signaling in intact lung remains limited. 20-hydroxyeicosatetraenoic acid (20-HETE) is an endogenous fatty acid that mediates signaling for a number of key physiologic endpoints in the pulmonary vasculature, including cell survival and altered vascular tone. We used confocal microscopy to identify enhanced reactive oxygen species (ROS) production in endothelial cell (EC)s in intact lung evoked by two stable analogs of 20-HETE, 20-5,14-HEDE (20-hydroxyeicosa-5(Z),14(Z)-dienoic acid) and 20-5,14-HEDGE (N-[20-hydroxyeicosa-5(Z),14(Z)-dienoyl]glycine). These analogs generated increased ROS in cultured pulmonary artery endothelial cells as well. 20-HETE analog treatment decreased apoptosis of pulmonary tissue exposed to hypoxia-reoxygenation (HR) ex vivo. Enhanced ROS production and apoptosis were confirmed by biochemical assays. Our studies identify physiologically critical, graded ROS from ECs in live lung tissue ex vivo treated with 20-HETE analogs and protection from HR-induced apoptosis. These methodologies create exciting possibilities for studying signaling by stable 20-HETE analogs and other factors in pulmonary endothelial and other lung cell types in their native milieu.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 318, Issue 16, 1 October 2012, Pages 2143-2152
Journal: Experimental Cell Research - Volume 318, Issue 16, 1 October 2012, Pages 2143-2152
نویسندگان
Elizabeth R. Jacobs, Sreedhar Bodiga, Irshad Ali, Aaron M. Falck, John R. Falck, Meetha Medhora, Anuradha Dhanasekaran,