کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2130977 | 1086614 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intermediate monomer-dimer equilibrium structure of native ICAM-1: Implication for enhanced cell adhesion
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کلمات کلیدی
FITCICAM-1GPiLFA-1SDF-1αLymphocyte function-associated antigen-1 - آنتی ژن 1 مرتبط با عملکرد لنفوسیتimmunoglobulin - ایمونوگلوبولینImmunoglobulin superfamily - خانواده فوقانی ایمونوگلوبولینintracellular domain - دامنه درون سلولیDimer - دیمرfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتintercellular adhesion molecule-1 - مولکول چسبندگی بین سلولی -1Cell adhesion - چسبندگی سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Dimeric intercellular adhesion molecule-1 (ICAM-1) has been known to more efficiently mediate cell adhesion than monomeric ICAM-1. Here, we found that truncation of the intracellular domain of ICAM-1 significantly enhances surface dimerization based on the two criteria: 1) the binding degree of monomer-specific antibody CA-7 and 2) the ratio of dimer/monomer when a mutation (L42 â C42) was introduced in the interface of domain 1. Mutation analysis revealed that the positively charged amino acids, including very membrane-proximal 505R, are essential for maintaining the structural transition between the monomer and dimer. Despite a strong dimer presentation, the ICAM-1 mutants lacking an intracellular domain (IC1ÎCTD) or containing R to A substitution in position 505 (505R/A) supported a lower degree of cell adhesion than did wild-type ICAM-1. Collectively, these results demonstrate that the native structure of surface ICAM-1 is not a dimer, but is an intermediate monomer-dimer equilibrium structure by which the effectiveness of ICAM-1 can be fully achieved.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 317, Issue 2, 15 January 2011, Pages 163-172
Journal: Experimental Cell Research - Volume 317, Issue 2, 15 January 2011, Pages 163-172
نویسندگان
Hyun-Mee Oh, Min-Sung Kwon, Hyang-Jin Kim, Byeong-Hun Jeon, Hye-Ran Kim, Hyang-Ok Choi, Bo-Ra Na, Soo-Hyun Eom, Nam Woong Song, Chang-Duk Jun,