کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2131870 1086663 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evidence for γ-actin as a Z disc component in skeletal myofibers
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Evidence for γ-actin as a Z disc component in skeletal myofibers
چکیده انگلیسی

We investigated the targeting of the γ-actin isoform in skeletal myofibers. For this purpose we used expression vectors to produce green fluorescent protein (GFP-) as well as myc-tagged γ-actin in rat flexor digitorum brevis myofibers. We found that the γ-actin fusion proteins accumulated into Z discs but not beneath the sarcolemma. Instead, the GFP-tagged skeletal muscle-specific α-actin isoform was preferentially incorporated into the pointed ends of thin contractile filaments. The localization pattern of the γ-actin fusion proteins was completely different from that of the dystrophin glycoprotein complex on the sarcolemma. The results emphasize the role of γ-actin as a Z disc component but fail to reveal an actin-based sub-sarcolemmal cytoskeleton in skeletal muscle cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 315, Issue 2, 15 January 2009, Pages 218–225
نویسندگان
, , , , ,