کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2131870 | 1086663 | 2009 | 8 صفحه PDF | دانلود رایگان |

We investigated the targeting of the γ-actin isoform in skeletal myofibers. For this purpose we used expression vectors to produce green fluorescent protein (GFP-) as well as myc-tagged γ-actin in rat flexor digitorum brevis myofibers. We found that the γ-actin fusion proteins accumulated into Z discs but not beneath the sarcolemma. Instead, the GFP-tagged skeletal muscle-specific α-actin isoform was preferentially incorporated into the pointed ends of thin contractile filaments. The localization pattern of the γ-actin fusion proteins was completely different from that of the dystrophin glycoprotein complex on the sarcolemma. The results emphasize the role of γ-actin as a Z disc component but fail to reveal an actin-based sub-sarcolemmal cytoskeleton in skeletal muscle cells.
Journal: Experimental Cell Research - Volume 315, Issue 2, 15 January 2009, Pages 218–225