|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|22748||43380||2016||12 صفحه PDF||سفارش دهید||دانلود رایگان|
• Stress-induced cytokinin synthesis improved yield of maize grown under water-deficit.
• Maize plants expressing pSARK::IPT displayed similar development as that of control plants.
• pSARK::IPT maize plants showed enhanced photosynthetic activity under water deficit.
• The source/sink relationships of pSARK::IPT plants under water-deficit remained similar to control plants.
• Water deficit treatment not affected floral synchrony or the kernel set of pSARK::IPT plants.
Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photosynthetic activity and improved tolerance to abiotic stress. The aim of this study was to generate and characterize transgenic plants of maize (Zea mays L.) transformed with the IPT gene sequence under the regulation of SARK promoter (protein kinase receptor-associated senescence). Three independent transgenic events and their segregating null controls were evaluated in two watering regimes (WW: well watered; WD: water deficit) imposed for two weeks around anthesis. Our results show that the WD treatment induced IPT expression with the concomitant increase in cytokinin levels, which prolonged the persistence of total green leaf area, and maintained normal photosynthetic rate and stomatal conductance. These trends were accompanied by a minor decrease in number of grains per plant, individual grain weight and plant grain yield as compared to WW plants. Plants expressing the IPT gene under WD had PGR, anthesis and silking dates and biomass levels similar to WW plants. Our results demonstrate that expression of the IPT gene under the regulation of the SARK promoter helps improve productivity under WD conditions in C4 plants like maize.
Journal: Journal of Biotechnology - Volume 220, 20 February 2016, Pages 66–77