کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
23318 43431 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Adaptation of Cupriavidus necator to conditions favoring polyhydroxyalkanoate production
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Adaptation of Cupriavidus necator to conditions favoring polyhydroxyalkanoate production
چکیده انگلیسی

The fatty acid (FA) composition of the bacterial membrane of Cupriavidus necator DSM 545 was assessed during the time course of two-stage fed-batch cultivations for the production of short-chain polyhydroxyalkanoates (PHA). Changes in the relative proportion of straight, methyl and cyclopropyl saturated, unsaturated, hydroxy substituted and polyunsaturated FA were observed, depending on the C sources and cultivation conditions used to favor the synthesis of poly(3-hydroxybutyrate) (P(3HB)), poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB)) or poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate) (P(3HB-4HB-3HV)), under N limiting conditions. The relative percentage of each FA class was studied using glucose or waste glycerol (GRP), as main C source for P(3HB) production. The FA profile was also assessed when GRP was used together with i) γ-butyrolactone (GBL) (precursor of 4HB monomers) for P(3HB-4HB) synthesis and ii) GBL and propionic acid (PA) (3HV precursor) to yield P(3HB-4HB-3HV). The effect of GBL and PA utilization as PHA monomer precursors on the FA profile of the cell membrane was studied under two different dissolved oxygen concentrations (DOC).


► Synthesis of PHAs could be influenced by carbon sources and cultivation conditions.
► The proportion of the cellular membrane fatty acids changed in response to conditions.
► Fatty acids allowing stretching of the membrane were produced during PHA accumulation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 164, Issue 2, March 2013, Pages 309–317
نویسندگان
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