|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|23631||43459||2012||8 صفحه PDF||سفارش دهید||دانلود رایگان|
A comparative study was made of Mung bean epoxide hydrolases-catalyzed asymmetric hydrolysis of styrene oxide to (R)-1-phenyl-1,2-ethanediol in an n-hexane/buffer biphasic system containing various hydrophilic ionic liquids (ILs). Compared to the n-hexane/buffer biphasic system alone, addition of a small amount of hydrophilic ILs reduced the amount of non-enzymatic hydrolysis, and improved the reaction rate by up to 22%. The ILs with cation containing an alkanol group, namely [C2OHMIM][BF4] and [C2OHMIM][TfO], and the choline amino acid ILs [Ch][Arg] and [Ch][Pro] were found to be the most suitable co-solvents for the reaction, owing to their good biocompatibility with the enzyme, which led to high initial rates (0.99–1.25 μmol/min) and high product e.e.s (95%). When substrate concentration was around 30 mM, where optimal performance was observed with the IL-containing systems, the product e.e. was improved from 90% without ILs to ≥95% in the presence of ILs.
Figure optionsDownload as PowerPoint slideHighlights
► Addition of a small amount of hydrophilic ILs improved Mung bean EHs-catalyzed hydrolysis of SO.
► ILs effectively reduced the amount of non-enzymatic hydrolysis of SO.
► Diverse ILs showed different biocompatibility to EHs and effects on the reaction.
► ILs substantially increased the thermostability of Mung bean EHs.
► ILs affected the partition coefficients of the substrate between the two phases.
Journal: Journal of Biotechnology - Volume 162, Issues 2–3, 31 December 2012, Pages 183–190