Calorimetric control of the specific growth rate during fed-batch cultures of Saccharomyces cerevisiae

The specific growth rate of a Saccharomyces cerevisiae strain with glucose as limiting C-source was estimated from the measured heat flow produced by the cells. For the cultivation a standard 30 l laboratory bioreactor was used, which was extended in such a way that heat balancing is possible. The feed rate was adjusted by a feedforward/feedback controller such that the specific growth rate was kept on the desired set-point value. On the basis of experimental investigations it was demonstrated that the specific growth rate can be controlled at a given set point value below the critical value to prevent the production of growth-inhibitory ethanol due to the Crabtree effect. With this control strategy high biomass concentrations of more than 110 g l−1 can be obtained.

► A standard 30 l laboratory bioreactor was extended in such a way that heat balancing is possible.
► The specific growth rate of a Saccharomyces cerevisiae strain can be estimated from the measured heat flow produced by the cells.
► The specific growth rate can be controlled at a given set point value below the critical value to prevent the production of growth-inhibitory ethanol due to the Crabtree effect.