Broad HIV-1 neutralizing antibody response induced by heterologous gp140/gp145 DNA prime-vaccinia boost immunization

► We developed a novel immunization strategy for HIV vaccine. ► Heterologous gp140/gp145 immunization induces potent neutralizing antibodies. ► Heterologous gp140/gp145 immunization induces cross-reactive neutralizing antibody. ► This strategy can be applied for vaccine development against genetic variance pathogens.