کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2402992 | 1102871 | 2011 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Borrelia burgdorferi BBA52 is a potential target for transmission blocking Lyme disease vaccine Borrelia burgdorferi BBA52 is a potential target for transmission blocking Lyme disease vaccine](/preview/png/2402992.png)
The surface-exposed antigens of Borrelia burgdorferi represent important targets for induction of protective host immune responses. BBA52 is preferentially expressed by B. burgdorferi in the feeding tick, and a targeted deletion of bba52 interferes with vector–host transitions in vivo. In this study, we demonstrate that BBA52 is an outer membrane surface-exposed protein and that disulfide bridges take part in the homo-oligomeric assembly of native protein. BBA52 antibodies lack detectable borreliacidal activities in vitro. However, active immunization studies demonstrated that BBA52 vaccinated mice were significantly less susceptible to subsequent tick-borne challenge infection. Similarly, passive transfer of BBA52 antibodies in ticks completely blocked B. burgdorferi transmission from feeding ticks to naïve mice. Taken together, these studies highlight the role of BBA52 in spirochete dissemination from ticks to mice and demonstrate the potential of BBA52 antibody-mediated strategy to complement the ongoing efforts to develop vaccines for blocking the transmission of B. burgdorferi.
► BBA52 is an outer membrane and surface-exposed protein of Lyme disease pathogen.
► BBA52 antibody lacks bactericidal activities in vitro but binds to pathogen surface.
► BBA52 vaccinated mice are less susceptible to infection with Lyme disease pathogen.
Journal: Vaccine - Volume 29, Issue 48, 8 November 2011, Pages 9012–9019