کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2403534 1102913 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease
چکیده انگلیسی

In this study, West Nile virus (WNV) envelope (rE) protein and its domain III (rDIII) were efficiently expressed in a cost-effective system based on insect larvae as non-fermentative living biofactories. Mice immunized with the partially purified rE or rDIII elicited high antibodies titers that neutralized viral infectivity in cell culture and in suckling mice. All vaccinated animals were fully protected when challenged with neurovirulent WNV NY99. Passive transfer of protective antibodies from immunized mothers to their offspring occurred both by transplacental and lactation routes. These results indicate that the insect-derived antigens tested may constitute potential vaccine candidates to be further evaluated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 29, Issue 9, 17 February 2011, Pages 1830–1835
نویسندگان
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